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Tracheotomised and ventilated Balb/c mice were submitted to a controlled hemorrhagic shock to reach a MAP level of 40 mmHg during sixty minutes.

AS mice were exposed to noise (300 Hz–70 dB) during 24hs and randomized into: A) Acute effects group: mice were killed after AS; L) Late effects group: mice were killed 3 weeks after AS and O) Over-exposed effects group: mice were submitted to AS once a week during a month and killed.

The non-stressed control mice were submitted to the same handling at the same time except for the immobilization procedure.

After random selection, rats and Swiss mice were submitted for eight weeks to a control or high-fat (HF) diet, as presented in Table 1.

Transgenic mice were submitted to chronic psychosocial stress using a well established experimental paradigm that serves as a model for depression[18].

For 6 consecutive days before inoculation, all mice were submitted to open field (days 1 to 3), LCE (days 4 and 5), and dark/light box (day 6) to obtain a baseline curve for these tests.

Approximately 5  μg of total RNA from transgenic and control mice was submitted to the Beijing Genomics Institute (BGI) for Solexa sequencing.

Heart tissue from sham and PM1-treated mice was submitted to all the procedures above described for lungs, and homogenates tested for HO-1, Caspase8-p18, NF κB-p50, E-selectin, iNOS, and MPO (anti-HO-1 1 : 200, anti-Casp8-p18 1 : 200, anti-NF κB-p50 1 : 200, anti-E-selectin 1 : 200, anti-iNOS 1 : 200, anti-MPO, all by Santa Cruz).

Each mouse was submitted to one injection of 2 × 106 cells in 500 μl Matrigel (Becton Dickinson).

DNA from the same mouse was submitted to GeneSeek (Lincoln, NE) for hybridization on the Mega Mouse Universal Genotyping Array (MegaMUGA), which provides 77,800 SNP markers built on the Illumina Infinium platform.

At 8 weeks of age, mice were assigned to the following groups: wild-type (WT) mice which were submitted to sham operations (WT SHAM); WT mice with chronic kidney disease (WT CKD); Apolipoprotein-E knockout (Apo-E KO) mice which were submitted to sham operations (Apo-E KO SHAM); and Apolipoprotein-E knockout mice with CKD (Apo-E KO CKD).