Of the septic animals surviving to 24 h, 38% of the mice were scored as mild and 62% as severe at this time point, while 33% of the rats were scored mild and 66% severe.
The mice were scored upto day 311 (Fig. 5A).
SI's from at least five mice were scored per data point.
The moribund mice were scored as "dead", and were euthanized using carbon dioxide.
The mice were scored for their gross appearance every 24 h for 21 days, after which they were sacrificed.
Furthermore, KO mice were scored significantly higher than WT in the first 15 min following KA injection, as well as throughout the 3h monitoring period (Figure 4B).
The NPs and vehicle transplanted mice were scored daily for clinical signs of EAE during a 43 day period (Fig. 2).
To achieve congruent scoring criteria, a separate cohort of mice were scored by both observers until they were consistent in their criteria prior to working with the mice used for these experiments.
To obtain a quantitative measure of the effect of treatment on histopathology, sections of both hind paws of all mice were scored for the severity of joint damage, extent of inflammation and bone erosion using an arbitrary scale by independent investigators 'blinded' to mouse identity.
To eliminate any diagnostic bias, mice were scored blindly.
Recipient mice were scored as engrafted if they displayed >0.1% Ptcrpb cells in multiple lineages.
