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Then, all the mice were immersed vertically 4 h every day in a 21 ± 2 °C water bath cages.
The WIRS model, in which Blab/c mice were immersed in 21 ± 2 °C water for 4 h each day for 14 days, was established.
When the tails of normal mice were immersed in exceedingly hot water, they quickly withdrew them, whereas mice without TRPV1 reacted in a more leisurely fashion.
The proximal aortas of apoE−/− mice were immersed in ice-cold 4 % paraformaldehyde for 30 min.
The remnants of embryos and three newborn mice were immersed in an acid-alcohol fixative comprising 96% ethanol, 1% acetic acid, and 3% distilled water for perlecan, laminin, and type IV collagen IHC [ 15].
Burn injury was produced with 80°C water, and mice were immersed for 10 seconds with a burn template so that the dorsum was exposed to hot water through a 2 × 3 cm window.
Similar(54)
15 min before the experiment. 1 to 2 cm of the tail of mice was immersed in warm water kept constant at 52 ± 1°C.
After that, the tail and hind legs of each mouse were immersed for 1 h in water containing 50 cercariae.
The brain sections (10 to 12 sections of the PVN region from each mouse) were immersed in guinea pig anticorticotropin-releasing factor (CRF) (1 : 500; Bachem Inc., Torrance, CA, USA) or rabbit anti-GR (1 : 100; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) at 4°C for 24 h.
One-half of the kidney from each mouse was immersed in TBS tissue freezing medium (Triangle Biomedical Sciences, Durham, NC, 27705, USA) and snap frozen in methanol-dry ice.
After dissection, left hemispheres of mice brains were immersed in 4% paraformaldehyde for 2 hours and then transferred to a solution of 0.95 mM potassium dichromate in 80 mM formaldehyde diluted in distilled water for 48 hours.
Related(20)
mice were resulted
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mice were brought
specimens were immersed
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mice were engaged
mice were dipped
mice were soaked
mice were split
mice was immersed
mice were administered
mice were sacrificed
mice were chosen
mice were hunted
mice were injected
mice were challenged
mice were tested
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