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The hind paws of mice were dipped in black India Ink and mice were allowed to walk down a corridor lined with white paper.
The tails of mice were dipped in water maintained at 55°C, while holding animals in vertical position.
For the gait analysis on the footprint assay, the paws of the mice were dipped in non-toxic water-soluble paints.
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The lower 3 cm portion of the tail of mice was dipped in a water bath (Le-Chatelier Thermostat, UK) maintaining at temperature of 55 ± 0.5°C.
For footprint analysis, each paw of the mouse was dipped in a different color of edible paint and the mice were habituated for one day to the testing chamber, a cardboard tunnel with a wide filter paper pad on the floor.
To obtain the footprints, mouse paws were dipped in nontoxic water-based food dye.
The hind paws of mice at 5-6 moldhs old were dipped into black non-toxic paint.
Their hindpaws were dipped in blue fountain pen ink.
Control seedlings were dipped in sterile distilled water.
Samples were dipped up and down 10 times during incubation.
The animals were marked on both the hind paws (right and left), just beyond the tibiotarsal junction so that the mouse paw was dipped to the same level in the water bath every time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com