Sentence examples for mice was added from inspiring English sources

In animal studies impaired capacity to reduce surface tension occurred when BAL fluid from RSV-infected BALB/c mice was added to calf lung surfactant extract [ 25].

A standard serum composed of a mixture of sera from the arthritic mice was added to each plate in serial dilutions and a standard curve was constructed.

A standard serum composed of a mixture of sera from arthritic mice was added to each plate in serial dilutions and a standard curve was constructed.

This medium was made sterilized by pressure passage through 0.22  μm membrane filters (Millipore, Germany) and then 10 mL (10%) urine (filtered and autoclaved of both sheep and mice) was added.

Briefly, a single-cell suspension of splenocytes harvested from each group of vaccinated mice was added to microtiter wells that were pre-coated overnight at 4 °C with anti-mouse-IFN-gamma antibody (BD Biosciences PharMingen).Lymphocyte stimulation was performed in triplicates at 37 °C for 72 h, with the E5 protein.

DBA/1J mice were treated with either 500 µL PBS control or 2 mg/kg FK506 (Astellas) subcutaneously for 14 days, or 0.5 × 10 FKDC-CII (prepared as above) intravenously on days 7 and 14. 4 hr after the last treatment, serum from individual mice was added to CD4+ splenocytes stimulated with CD3/28 dynabeads or media control for 4 hr at 37°C. mRNA was isolated and qRT-PCR performed.

For bronchoalveolar lavage assays, an additional 5 mice were added for each group.

Purified Gr-1+CD11b+ cells from IL-6-treated mice were added to CFSE-labeled T cells stimulated with anti-CD3.

In some wells, FACS-sorted CD4+6.5+CD25− or CD25+ T cells obtained from dtg mice were added to CD4+6.5+CD25− from stg mice at a ratio of 1∶1.

Splenocytes from individual mice were added in duplicates and incubated for 48 h at 37°C, 5% CO2 with 5 µg of OVA.

Splenocytes isolated from these mice were added in serial dilution to DNA-coated plates and incubated for 12 hours at 37°C.