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Detection of KCC2 was done on 30 μm coronal cryosections from fixed brains (P6 mice, fixed with 4% PFA).

Tissues were removed from treated mice, fixed with 4% paraformaldehyde and embedded with paraffin.

Skin was removed from the mice, fixed with 10% phosphate-buffered formaldehyde (pH 7.2), and prepared for histological examination.

All organs were removed from the mice, fixed with 4% phosphate-buffered formaldehyde (pH 7.2) and prepared for histological examination.

To identify the skeletal muscle vascular phenotype, pectoralis, triceps, tibialis anterior, gastronemous and quadriceps tissue sections from 6 12 month old (MO) C57BL6 mice fixed with 2% Paraformaldehyde (PFA) were stained for smooth muscle actin (SMA), Sca-1 and CD34, then costained for CD31.

The probes were subjected to in situ hybridization using 20 µm brain sections of mice fixed with 4% paraformaldehyde/PBS by perfusion as described previously (6).

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Intact PC12 cells expressing Q23 or Q74 growing on collagen IV-coated plates and the sections of brain tissues from wild-type mice and HD transgenic mice were fixed with a solution containing 2% (v/v) glutaraldehyde and 4% formaldehyde in 0.1 M sodium cacodylate, pH 7.4, for 15 min at room temperature.

The fluorescence of FITC was not detected when mice was fixed with PFA fixative before the perfusion of FITC-containing saline.

The lung tissues removed from the infected mice were fixed with 2.5% glutaraldehyde and post-fixed in 1% osmium tetroxide.

Briefly, adult mice were fixed with paraformaldehyde; the muscles were removed, post-fixed, rinsed and mounted on slides.

Muscles were dissected, snap-frozen for biochemistry (FVB/N mice), or fixed with 4% buffered paraformaldehyde for 30 min for NMJ analysis (Thy1-YFP mice).

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