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The specimens fixed with the combination of plates with the four-holed reversed L-shaped and with two-holed rectangular shaped; or with six-holed anatomical T-plates, showed significantly better stability than those of others.
Images of the specimens fixed with the 3 different systems are shown in Figure 3.
Surgeons harvest LNs from the fresh specimens immediately after surgery in Japan, while the pathologists from the specimens fixed with formalin after surgery in other countries [ 3].
Surgeons harvest LNs from fresh specimens immediately after surgery in Japan, whereas the pathologists generally harvest the nodes from specimens fixed with formalin after surgery in other countries.
However, specimens fixed with unicortical locked plating showed decreased stiffness during torsional loading when compared to bicortical LCDCP plate fixation of the osteotomized specimens.
The HER2 status of these tumor tissues were assessed by IHC and FISH, using specimens fixed with 10%% NBF for 24 h immediately after resection of the tumors.
Serial 5-mm-thick tissue sections of the entire tumour were prepared from the resected specimens fixed with 10% buffered formalin, embedded in paraffin and stained with haematoxylin and eosin (H&E).
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The mean percent area of X-gal staining in the specimen fixed with acetone was more than twice as large as those with other fixatives (Fig. 1e, p < 0.01).
The camera and cast specimen were placed in a standardized position using a scale, with the cast specimen fixed with an adjustable clamp and ring stand at a set distance from the camera.
All surgically resected tumor specimens and biopsy specimens were fixed with 10%% formalin and embedded in paraffin.
All surgically resected tumour specimens and control specimens were fixed with 10% formalin and embedded in paraffin and obtained from the archives of our institute.
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