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Exact(16)
Briefly, tissue biopsies of ∼50 mg were homogenised and the microsomal protein fraction was isolated by ultracentrifugation.
For this purpose neocortical brain tissue samples (70 100 mg) were homogenised in 1 ml Trizol reagent (Invitrogen, Karlsruhe, Germany) and mRNA was isolated according to the instructions of the product supplier.
After ALA or He ALA exposure, explants of 50 mg were homogenised in a 4 : 1 solution of ethyl acetate-glacial acetic acid mixture.
Tissue samples were weighed, and then sectioned with a scalpel, and 20 30 mg were homogenised using the Tissue-Tearor (BioSpec Products, Bartlesville, OK, USA).
Fresh frozen breast tumour tissues (about 30 mg) were homogenised with a microdismembrator (B Braun, Melsungen, Germany), and genomic DNA was then isolated with the SV Genomic DNA Purification System Promega Corporationn, Madison, WI, USA).
Hippocampal punches, weighing 20 30 mg were homogenised in 100 μl of lysis buffer (50 mM Tris HCl, 150 mM NaCl, 1% Triton ×10) with protease and phosphatase inhibitors.
Similar(44)
Chopped needle tissue (300 mg) was homogenised with a mortar and pestle under liquid nitrogen, placed into a 2 mL tube and 1 mL of pre-warmed (65°C) CTAB buffera added.
Tissue of 50 mg was homogenised with CHAPS lysis buffer.
A frozen biopsy (<20 mg) was homogenised by a Heidolph Diax 600 mixer.
Frozen liver tissue (~100 mg) was homogenised in 1.6 ml phosphate-buffered saline and protein concentration was determined using the method of Lowry [ 17].
The rest of the sample, mean 65.8 mg, was homogenised thoroughly in distilled water (100 mg wet tissue 6 ml−1 distilled water), and centrifuged at 3000 r.p.m. (1000 g) for 5 min.
Related(20)
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