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Starchy endosperm (5 mg) was ground with a mortar and pestle and the powder was boiled in 1.5 ml of methanol for 10 min.
The dried material (200 mg) was ground to a powder and extracted in 10 mL of distilled water for 2.5 h.
Dry plant material (∼100 mg) was ground to a fine powder in a mortar and extracted with 80%% methanol.
Dry leaf material (20 50 mg) was ground using a Mini Beadbeater 8 (BioSpec Products, Bartlesville, OK).
Muscle (25 mg) was ground in liquid nitrogen, and mixed muscle protein was precipitated using 1 M perchloric acid and the pellet washed twice with 70% ethanol.
Xenograft tumour material (∼150 mg) was ground under liquid nitrogen in a pestle and mortar then a dual phase extraction was used, as described above.
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Rosiglitazone tablets (Avandia™, 4 mg) was grounded and dissolved in 0.1% DMSO and given to mice bearing H441GL tumor via daily gavage at a concentration of 10 mg/kg/d.
The CE MS samples weighing approximately 300 mg wet weight was ground using a multi-bead shocker (model MB755U; Yasui Kikai, Osaka, Japan) with frozen metal granules, and 1 mL methanol containing internal standards (300 μmol L−1 each of l-methionine sulfone and 2-morpholinoethanesulfonic acid (MES)) was added.
Up to 30 mg of dry material was ground for 2 min in a TissueLyser mixer-mill disruptor (Qiagen, California, USA) using tungsten beads.
Approximately 200 mg of potassium bromide powder was ground.
The sample (2 mg) was finely grounded with purified potassium bromide (200 mg; to remove scattering effects from large crystals).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com