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Compound 9 (5 mg) was isolated from fraction A1 by preparative TLC (CHCl3 MeOH, 30:1).
Maculine (3, 1.5 mg) was isolated from A2 eluted with hex/EA (95:5).
Fraction C2 (500 mg) was isolated by silica gel CC using petroleum ether/Me2CO (6:1) to afford several subfractions, and compound 6 (13 mg) crystallized from one subfraction.
Compound 10 (RT: 14.0 min; 2 mg) was isolated from another MPLC fraction (RT: 64.0 67.0 min; 1.8 g) by RP HPLC (linear gradient from 90 to 100%% B1 in 20 min, followed by 15 min isocratic conditions).
Fraction C6 (300 mg) was isolated by Sephadex LH-20 (MeOH) and then purified by HPLC (MeCN H2O (v/v) from 3 7 to 4 6 in 25 min) to afford 5 (6.8 mg) and 7 (3.4 mg).
Soy (100 mg) was isolated according to the supplied protocol.
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Compound 1 (70.0 mg) and 4 (18.2 mg) were isolated from F1.7 by using a reverse phase C18 column eluted with acetone water (3:2 v/v).
Using the same column chromatography as above, saccharides 2 (10.5 mg) and 3 (4.8 mg) were isolated from fraction IV (30 mg; after 2.8 L of 5% ethanol elution, 0.3 –1.1 L of 10% ethanol elution) and fraction IX (80 mg; after 5.6 L of 5% ethanol elution, 2.5 –2.9 L 10% ethanol elution) as white powders, respectively.
Bacterial organisms identified as Mycoplasma gallisepticum (MG) were isolated from lesions of infected birds.
The α-MG was isolated and purified by repetitive column chromatography as previously described [ 26].
With the development of MGs, a new concept of interconnected microgrids system (IMS) (or microgrid cluster) is introduced which considers several MGs exchanging energy with each other even when the MGs are isolated from the utility grid.
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