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After being immersed in liquid nitrogen and ground into fine tissue powder in a mortar, tissue samples (about 50 mg) were ground with PBS containing Protease Inhibitor Cocktail II Upstatee, Lake Placid, NY, USA).
The frozen plant tissues (50 100 mg) were ground to a fine powder in liquid nitrogen using a micro-pestle.
Commercial sulfur powder (200 mg) and GHCS (100 mg) were ground thoroughly using mortar and pestle to make a homogenous mixture.
Whole grains of each line (approximately 100 ~ 150 mg) were ground in a capsule with ball bearing using an ESPE CapMix™ (model 3 M, AU).
The seed samples (100 mg) were ground in liquid nitrogen and the RNA was isolated using the Invisorb Spin Plant-RNA Mini Kit (Invitek), according to the manufacturer's specifications.
Hair samples (ca. 70 mg) were ground with a ball-grinder (Retsch, Germany) for 10 min.
Similar(36)
Starchy endosperm (5 mg) was ground with a mortar and pestle and the powder was boiled in 1.5 ml of methanol for 10 min.
The dried material (200 mg) was ground to a powder and extracted in 10 mL of distilled water for 2.5 h.
Dry leaf material (20 50 mg) was ground using a Mini Beadbeater 8 (BioSpec Products, Bartlesville, OK).
Dry plant material (∼100 mg) was ground to a fine powder in a mortar and extracted with 80%% methanol.
Xenograft tumour material (∼150 mg) was ground under liquid nitrogen in a pestle and mortar then a dual phase extraction was used, as described above.
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