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Additional methods and sequence accession numbers are presented elsewhere (Additional file 1).
The decreasing cost of high-throughput sequencing has led to a proliferation of template preparation methods and sequence data (Sims et al. 2014).
For example, "hotspot" and "coldspot" regions have been identified using statistical methods and sequence information from large number of isolates [ 14].
Moreover, sequence-based identification techniques were found to be more efficient than other phenotypic methods, and sequence analysis of the ITS region provided a higher percent of accurate identification [ 25].
However, we find it important that the identification of FUS and EWS binding downstream the poly(A -signal of A -signalubset of transmalled target genesubsetspectively of peak calling metranscribedequence datarget, indicates an overseen putative regenesory mechanirrespectivelyargeting of FUS and EWS with peakntial implicalling for regulation at the RNA level.
With the advent of new molecular techniques, we have been able to investigate bacterial diversity in different microhabitats using molecular fingerprinting methods and sequence analysis of microbial small subunit ribosomal (r) RNA genes (16S rRNA) and other universal targets (such as cpn60) [ 13, 36, 37].
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This provided an excellent opportunity to compare the inter-laboratory reliability using two different target sequencing methods and sequencing platforms.
Due to the variety of image acquisition methods and sequences, especially in MRI, it is crucial to store detailed image acquisition parameters along with the image data.
Protein function assignment methods can be divided into two main categories - structure-based methods and sequence-based methods.
These methods can be classified into two categories: sequence-sequence alignment methods and sequence-structure alignment methods [ 13].
Several methods have been proposed to screen proteolytic (including keratinolytic) enzymes, including keratin-baiting, plate screening, spectrophotometric methods, and sequence-based amplification.
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