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The present study provides a methodologic approach and sequence information to carry out a large-scale screening of mating-type loci organization in Z. sapae and Z. rouxii.
In the present study, the GWAS mapping approach and sequence based SNP maps with a large and diverse sample of soybean germplasm collection were used to identify the quantitative trait loci associated with the resistance to SCN Hg Type 0 (race 3) and Hg Type 1.2.3.5.7 (race 4).
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Thus, by combining both text mining candidate selection approach and sequence-based classifier, a novel hybrid strategy is proposed for gene priority with a specific function protein.
Using a comparative genomics approach and sequence-based neutrality test, Thompson et al. (2004) reported evidence of positive selection on the derived allele of the functional CYP3A5*3 in non-African populations and significant correlation of its allelic frequency with distance from the equator.
To identify the responsible mutations, we used a candidate approach and sequenced the coding sequences of several connexin genes.
Therefore, we took a candidate gene approach and sequenced the exonic regions of Cbr-lin-39 in bh20 animals.
Genomic DNA from Pa ATCC43949 was sequenced by a shotgun approach and sequences were assembled using the Phusion assembler [ 18], based on read pair information.
In addition, one 5 ng bulk RNA as a control was amplified by a tube-based approach and sequenced into both SE50 (8 million reads) and PE91 (46 million reads).
The nuclear loci consisted primarily of intronic regions with primers designed using an exon-primed intron spanning approach and sequences from the chicken and zebra finch genome (following [ 46]).
The choice between a whole-genome shotgun sequencing approach and sequencing reduced representation libraries was extensively discussed during the Human Genome Project (Lander et al., 2001; Venter et al., 2001), and the former became the dominant technology as the sequencing throughput rapidly increased, rendering library techniques to prepare data for the latter approach relatively expensive.
Clayton-Smith et al identified KAT6B, a histone acetyltransferase, as the causative gene for SBBS by a whole-exome sequencing approach and reported sequence variants in 13 individuals.
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