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For the purpose of enrichment efficiency, we compared the 6 matched samples, where were independently enriched, as described in methods, and sequenced on SOLiD 3.0 platform octet slides (18 octets "spots" in total).
The PCR products were purified as described in Materials and Methods and sequenced (GeneWiz).
The libraries were processed as described in the Methods and sequenced in Illumina GA.
An initial sampling of RVFV PCR products was cloned by using the above-mentioned methods and sequenced for confirmation.
The dissected bands were purified by standard methods and sequenced on a 3500 genetic analyzer which is mentioned previously.
Sample DNA was extracted from whole blood using standard methods and sequenced at Complete Genomics (Mountain View, CA, USA) using unchained combinatorial probe anchor ligation chemistry on self-assembling DNA nanoballs (DNBs) (43).
Similar(54)
These methods can be classified into two categories: sequence-sequence alignment methods and sequence-structure alignment methods [ 13].
Libraries of 22 single cells were constructed using Smart-seq2 method and sequenced.
For the development of sequence characterized amplified region (SCAR) markers, a 747 bp amplicon was generated by the ISSR method and sequenced.
Sequence-PCR products were cleaned up using the ethanol/EDTA precipitation method and sequenced bi-directionally on an ABI 3130×l Genetic Analyzer (Applied Biosystems Inc., CA, USA).
This pool was amplified using the Lib-A method and sequenced on a 454 GS Junior system (Roche) at Landcare Research, Auckland.
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