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A new method of fluorescence detection that promises to increase sensitivity by 20- to 1000-fold is described.
The rate of subunit exchange was measured by the method of fluorescence resonance energy transfer (FRET).
Using the acceptor photobleaching method of fluorescence resonance energy transfer (FRET), the nuclear RanGTP level was measured as the relative percentage of FRET efficiency.
A method of fluorescence TdT-Mediated dUTP Nick-End Labeling (TUNEL) (C1086, Beyotime Inc., Jiangsu, China) was used to measure cellular programmed death.
In the case of the 50 SH2 domains and 192 peptides included in this study, we confirmed 60 interactions by the orthologous method of fluorescence polarization.
Recent studies have described a method of fluorescence in situ hybridization (FISH) that is faster and more reliable than comparable techniques [ 11- 14], which can also be applied to paraffin-embedded tissue samples [ 15].
Similar(52)
In the present study, the simultaneous effects of both of the important anticancer drugs of 5-fluorouracil (5-FU) and oxali-palladium, on the structure of βLG were investigated using different spectroscopic methods of fluorescence and circular dichroism (CD) in combination with a molecular docking at two temperatures of 25 and 37 °C.
15, 16 Because longer wavelengths are used for two-photon excitation of fluorophores than for conventional methods of fluorescence imaging, this technique has many advantages, including better penetration depth, higher signal-to-background ratio and reduced tissue damage.
The method of synchronous fluorescence spectroscopy was carried out as described above.
A probable explanation for this observation is that a method of external fluorescence measurement on the intact lens cannot follow the increasing concentration of fluorophores because it is affected by increasing self absorption (figure 3 & 4) at λEx312 nm and λEx365 nm, respectively with age.
The data treatment method converts plots of fluorescence anisotropy vs. time that contain no peaks that are distinguishable from the noise of the anisotropy background into plots that show distinct fluorescence anisotropy peaks.
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