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In the present study, the simultaneous effects of both of the important anticancer drugs of 5-fluorouracil (5-FU) and oxali-palladium, on the structure of βLG were investigated using different spectroscopic methods of fluorescence and circular dichroism (CD) in combination with a molecular docking at two temperatures of 25 and 37 °C.
15, 16 Because longer wavelengths are used for two-photon excitation of fluorophores than for conventional methods of fluorescence imaging, this technique has many advantages, including better penetration depth, higher signal-to-background ratio and reduced tissue damage.
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A new method of fluorescence detection that promises to increase sensitivity by 20- to 1000-fold is described.
We conclude that there is some flexibility in sample processing methods for quantitative CD38 determination; however, it is preferable for a laboratory to employ one method of fluorescence quantitation calculation consistently because small differences are detected between different methods.
The rate of subunit exchange was measured by the method of fluorescence resonance energy transfer (FRET).
Using the acceptor photobleaching method of fluorescence resonance energy transfer (FRET), the nuclear RanGTP level was measured as the relative percentage of FRET efficiency.
In the case of the 50 SH2 domains and 192 peptides included in this study, we confirmed 60 interactions by the orthologous method of fluorescence polarization.
A method of fluorescence TdT-Mediated dUTP Nick-End Labeling (TUNEL) (C1086, Beyotime Inc., Jiangsu, China) was used to measure cellular programmed death.
They cite the presence of bloody ascites as a significant limitation to traditional imaging methods and describe their method of fluorescence lifetime as a way of overcoming this issue.
Recent studies have described a method of fluorescence in situ hybridization (FISH) that is faster and more reliable than comparable techniques [ 11- 14], which can also be applied to paraffin-embedded tissue samples [ 15].
A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons of the distribution and abundance of chromosomal sequences and improves the relatively low throughput of standard FISH methods.
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