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The model for cryopreservation was set up as a 2 × 2 × 2 × 5 design for cryoprotectant (glycerol or dimethylacetamide (DMA)), cryopreservation medium (Lake or ASG), method of dilution (fixed dilution volume versus fixed sperm concentration) and turkey line, respectively.
The cells prepared as above were plated in 96-well microplates using a conventional method of dilution [ 28].
Moreover, the method of dilution series results in only one value of efficiency for all dilutions, even though efficiency varies as the input concentration changes [ 19].
The generation time (TG) was determined by the formula: T G = Ln 2 / μ esp Eq (2) The minimum inhibitory concentration (MIC) of DBT was carried out using quantitative method of dilution in liquid medium, considered the reference to determine the sensitivity by S. marcescens against the different concentrations of DBT (0.0, 0.5, 1.0 and 2.0 mM).
The letter refers to the scale on which the dilution has been carried out: the letter D denotes the decimal method of dilution (that is, one part of liquid is added to nine parts of purified water, ethanol, glycerol or lactose); the letter C indicates the centesimal method (one part added to 99 parts of diluent).
Similar(54)
The advantage of using the method of infinite dilution as compared to isotopic dilution is that the influence of competitive ligands on ABA-X-BY630 dissociation kinetics can be investigated.
Finally, it should be noted that the frequently employed method of serial dilution, that is, multiple sequential dilution steps by the same factor, makes it hard to obtain as good coverage of a concentration range as in Figure 3C, which in turn often precludes acquisition of data good enough for quantitative analysis in structure activity relationships.
This, however, would have been a far less sensitive sampling method because of dilution and lack of microbial survival, and rectal sampling was therefore applied.
LM represents the Roman numeral for 50,000 (quinquaginta-millesimal-potency); the dilution factor is 1/50,000 instead of the customary method of 1/100 dilution (C potencies).
Cloning of transgenic parasites was performed by the method of limiting dilution in 96 well plates[35].
Cloning of transfected parasites was performed by the method of limiting dilution [64] in 96 well plates.
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