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The sequences were aligned using Clustal W Thompsonn et al. 1994) and analyzed using Neighbour-Joining method and p distance model.
A new intermediate refraction point (P_{1}^{prime }) is calculated between P 0 and P 2 using the dichotomy method, and P 1 is replaced by (P_{1}^{prime }).
P values for tumor growth were calculated by Kaplan-Meier Time to Progression method, and p values for two sample comparisons were calculated by two-sided Wilcoxon Rank-Sum test.
Models for use in OLS regression were selected using the backward elimination method, and p < 0.05 was considered statistically significant.
The survival rates were calculated with the Kaplan Meier method and P was determined by log-rank test.
The disease-specific survival analysis, based on death from vulvar cancer only, was performed using the Kaplan Meier method and P value computed by log-rank test.
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Having said that, some remarks and warnings should be given related mainly with the null hypothesis that underpin the method and p-value computation.
(In comparison, this method and p-value identifies 3271 EACS+B1 sites within the entire genome).
Survival curves were plotted by the Kaplan Meier method, and P-values were calculated with the log rank test.
Survival curve was plotted by the product-limit Kaplan Meier method, and P-values were evaluated by the Mantel log-rank test using SPSS software (Chicago, IL, USA).
Statistical analysis was done by using the Bonferroni method and P-value<0.05 was considered to be significant with * P<0.05, ** P<0.01 and *** P<0.001.
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