Sentence examples for membrane with primary from inspiring English sources

Immuno-blotting was carried out by incubating the membrane with primary antibody Anti-His Sigma-Aldrich diluted to 1 5000; then with the appropriate Horseradish Peroxidase conjugated secondary antibody diluted to 1 5000.

Blocking was carried out in 1×Rotiblock solution (Roth Chemicals) followed by incubating the membrane with primary antibody overnight at 4°C.

Incubate membrane with primary antibody diluted 1 10,000 overnight at 4°C. e. Wash membrane three times with TBST.

#Incubate membrane with primary antibody in blocking buffer for 2 hr at room temperature (RT) or overnight at 4°C (use manufacturer's suggested dilution in blocking buffer).

Protein was identified by incubating the membrane with primary antibodies, followed by horseradish peroxidase-conjugated secondary antibodies and an enhanced chemiluminescence solution (Pierce, Thermo Scientific).

j. #Incubate membrane with primary antibody in blocking buffer for 2 hr at RT or O/N at 4°C (use manufacturer's suggested dilution in blocking buffer).

In this study we developed liver organotypic co-culture systems by using synthetic and biodegradable membranes with primary human hepatocytes and human umbilical vein endothelial cells (HUVEC).

Equal loading and uniformity of protein transfer to the nitrocellulose membrane were verified by stripping and reprobing the membranes with primary antibodies specific to α-Tubulin or Actin.

Equal loading and uniformity of protein transfer to the nitrocellulose membrane were verified by stripping and reprobing the membranes with primary antibodies specific to α-Tubulin.

After blocking, we incubated membranes, with primary antibodies at 4°C over night.

We incubated membranes with primary serum at 4°C for 16 hr at a dilution of 1 1000 in blocking buffer.