This chapter will review the fundamental components of cardiomyocyte intercalated disc and transverse-tubule membranes with a focus on linking dysfunction in these membranes with human cardiovascular disease.
We have introduced a treatment procedure to promote regeneration of the tympanic membrane and closure of perforations using an atelocollagen sponge/silicone membrane combined with human basic fibroblast growth factor that promotes wound healing. 1, 2 This report describes epithelial pearl formation following our outpatient-based tympanic membrane regeneration therapy.
For transwell assays, T cells were incubated in starving medium for 5 h, incubated with plate-bound antibodies for 45 min then added to 3-µm pore transwell membranes coated with human fibronectin (2 µg/ml; Sigma-Aldrich), ICAM-1-Fc (5 µg/ml; R&D Systems) or uncoated.
In this study we developed liver organotypic co-culture systems by using synthetic and biodegradable membranes with primary human hepatocytes and human umbilical vein endothelial cells (HUVEC).
Oligoclonal fLC were analyzed by coating the membrane with anti-human kappa or lambda fLC (DAKO, Cat. No. A0100 and A0101, respectively), diluted to 20 mg/l in TBS.
Following three 5-min washes with TBS-Tween (0.05 %), the membrane was incubated with human fH at a concentration of 73 μg/ml in TBS for 14 h with gentle agitation.
Gärtner et al. seeded the porous membranes with undifferentiated human MSCs (hMSCs) isolated from Wharton's jelly [ 32].
This can be achieved either by masking of the anti- E. coli antibodies in human sera by addition of saturating concentrations of E. coli crude protein extracts and by blocking unwanted reactivity against E. coli by repeated incubation of membranes with the human serum.
Brain protein extracts were separated using 12% SDS-PAGE gels, blotted to PVDF membranes and probed with human Aß-antibody (clone 6E10) (Abcam, Cambridge, UK) according to standard immunoblotting procedures.
