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Since Hep could be employed as a medium for inducing the aggregation of positively charged Z-TPE-5, the designed Z-TPE-5 shows excellent fluorescence response to Hep through multiple electrostatic interactions with a detection limit of 1.53 ng/mL, which is far below than most of the reported method for the detection of Hep.
NSF-1-containing medium was more efficient than B27-supplemented medium for inducing sphere cells (data not shown).
Following cell confluence, the medium for inducing differentiation was used, and changed every 2 days until day 5 of differentiation.
Medium for inducing adventitious bud formation from explants was comprised of Murashige and Skoog (MS) basal elements, 3% sucrose, 0.3 mg/L IBA, and 0.01 mg/L NAA.
As seen in the diagram, EGM-2 MV SingleQuots Kit media (EBM-2) was the most effective medium for inducing the proliferation of HDMECs).
When the optical density of the bacterial culture at 600 nm reached 0.6 0.8, 0.1 mM isopropyl-β-d-thiogalactopyranoside was added to the medium for inducing enzyme expression.
Similar(54)
Part of the sterile seed pieces were also transferred into medium without phytohormones, for inducing embryo germination.
Generally, YPD media is used to generate biomass and is transferred into buffered minimal methanol medium (Invitrogen, USA) for inducing the AOX1 promoter to express the protein of interest.
We have developed a medium-based protocol for inducing the differentiation of bone marrow-derived multi-potent mesenchymal stromal cells (MSCs) into neurotrophic factor-secreting cells (MSC-NTF cells).
Probably, in the case of sandalwood SE suspension cultures in air-lift bioreactors, the inoculum carrying some amount of conditioned medium may be responsible for inducing the secretion of AGPs, santalols and phenolics.
Then, the cells were harvested by centrifugation, suspended in 30 mL BMMY medium and induced for the expression of recombinant xylanase by adding methanol to a final concentration of 1.0% at 24 h intervals at 30 °C for 72 h.
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