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Exact(5)
Temperature had significant positive influence (P < 0.05) on oxygen diffusivity estimated for different medium and analysis methods.
The culture medium used to grow the bacteria Brucella broth plus 10% FBS is a rich polyamine-containing medium and analysis by HPLC indicated that it contained 4.94 µM putrescine and 9.74 µM spermidine (Figure S1).
Then, 10 nM dexamethasone and 30 ng/ml LPS were added and cells incubated for another 20 h before harvesting the medium and analysis of IL-6 and TNF-α by ELISA BD Biosciencess, Allschwil, Switzerland).
NPAs were taken by deep nasal suctioning followed by the aspiration of 2 ml viral transport medium and analysis with an RSV rapid antigen test (Abbott TestPack RSV, Abbott laboratories) [ 26].
Structural stability of the plasmid was also examined by cultivation in selective medium for 25 generations, plating on solid medium and analysis of plasmid DNA from a number of colonies.
Similar(55)
Radiative transfer processes are reviewed next, including exchange between surfaces, the presence of a participative medium, and the analysis of combined heat transfer modes.
Transformation was confirmed by selection in erythromycin containing medium and plasmid analysis.
First, 30Kc19 protein was added to culture medium and immunocytochemical analysis was conducted using anti-30Kc19 rantibodytibody and rhodamine-conjugated anti-rabbit antibody.
After growing the fungi in FGY medium and comparative analysis of the extracts by HPLC-MS using purified pneumocandin B0 as a standard, the two major pneumocandins (A0 and B0) were produced by the G. lozoyensis WT strain as expected, but the pneumocandins were absent in the glnrps4 and glpks4 knockout mutants.
Coverslips were washed and mounted on glass slides with anti-fading medium for observation and analysis.
The medium recipe, transformation and analysis of the recombinants were carried out as per the kit manual.
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