Sentence examples for medium addition of from inspiring English sources

In the 2nd CM medium, addition of ethanol after the 24th hours decreased the cell density until the 72nd hours and remained stable until the end of the measurement.

Infection was performed by filtering the virus containing medium, addition of proteamine sulphate, and transferring of the virus containing medium to the target cells which were incubated further for 24 hrs.

In the normal borate medium, addition of CHX did not change the level of BOR1-GFP significantly over 2 hours.

In steroid deprived medium, addition of 17-beta-estradiol (E2, 10 nM) increased both TRPM8 mRNA expression and the number of cells which respond to Icilin, but failed to affect the Ca2+ entry amplitude.

Figure 2 shows that in the presence of Ca2+ in the medium addition of 5 mM KCl causes a relatively fast increase in intracellular K+ concentration similar in magnitude to that previously observed [ 17].

Penicillium purpurogenum IAM15392 was grown in 500-mL Erlenmeyer flasks containing 100 mL of basal medium or basal medium with addition of glutamine (f.c. 1 or 10 mM l-glutamine) for 72 96 h at 30 °C with shaking at 200 rpm.

Cells were washed once with 180 μl serum-free medium, before addition of new medium with or without test substances.

The cells were treated with the cytotoxic drug doxorubicin for 24 h followed by removal of medium and addition of fresh medium containing P276-00 for 96 h.

For experiments performed on adherent cells, stress was applied after 1 h 30 of recovery, by removal of the nutritive medium and addition of fresh medium containing the appropriate concentration of stressor.

The agar plate medium was the corresponding liquid medium with addition of 1.5% agar.

This leads to Zn2+ efflux due to lower extracellular Zn2+ concentrations in the culture medium (0.093 µg Zn2+/ml = 1.42 µM for Neurobasal medium with addition of B27, Pen/Strep and Glutamine measured by Plasma-Massspectrometry).