Sentence examples for medium supplement of from inspiring English sources

By supplying the cells with an alternative path for accumulation of Ile, Leu and/or Val, i.e., a medium supplement of the appropriate dipeptides, or by engineering the transport capacity for branched-chain amino acids, the expression levels could be increased several fold.

The tryptone soya agar (TSA) and tryptone soya broth (TSB) medium, supplement of a final concentration of 10% horse serum, 5% yeast extract (Becton, USA), and 0.05% NAD (Roche, China), were used to culture the H. parasuis, at 37°C in 5% CO2.

With the methods mentioned, the influence of 7 medium supplements on the growth of CHO cells in suspension was evaluated efficiently.

In this work, we employed Plackett-Burman design and SVM to explore the influence of 7 medium supplements on the growth of CHO cells in suspension.

Moreover, glycine was chosen as a medium supplement to improve the production of extracellular CBP21 or Chi92.

Adipogenesis was induced by replacing the culture media with adipocyte induction medium composed of culture medium supplemented with 1μM of insulin, 1 μM of dexamethasone and 1 μM of IBMX.

Subsequently, the inoculum (5%% v/v) was transferred to a 250-mL shake flask containing 100 mL operating volume of defined medium supplemented with glycerol of 30 g/L and yeast extract of 2 g/L.

Recycling was initiated by addition of complete medium supplemented with 1mg/ml of iron saturated holotransferrin (Sigma).

Human umbilical vein endothelial cells (HUVEC) (5×103cells/well) were added alone or mixed with mAb C7 CDR peptides in 100 µL of RPMI medium supplemented with 0.2% of FCS in each well.

Each petridish contained 20 mL of MS basal medium supplemented with a range of concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1 5.0 mg/L).

After transfer to 1 ml of medium supplemented with 5 mg of bovine serum albumin per ml and 15 mM NaHCO3, semen was allowed to exude (15 min at 37°C, 5% CO2) from three to five small incisions.