Sentence examples for mediating translational repression from inspiring English sources

We also used a gain of function approach to demonstrate miR-130a's role in mediating translational repression of FOG-2.

AGO1 is presented as a major antiviral slicer, but other AGO paralogs are likely involved, potentially also mediating translational repression [19] or DNA methylation in a sequence-specific manner [6].

To demonstrate the importance of the putative miR-130a binding site within the FOG-2 3'UTR for mediating translational repression, we generated a reporter construct with a mutation of the predicted binding site in the FOG-2 3' UTR (Fig. 3A).

Data suggest that miRNA binding sites within the coding region of a transcript are less effective at mediating translational repression.

DOI: http://dx.doi.org/10.7554/eLife.05005.006 10.7554/eLiFigure05.007 Figure 1 figure supplement 4. Inefficacy of non-canonical sites in mediating translational repression.

MicroRNAs (miRNAs) are small ~22 nt long non-coding RNAs which play important regulatory roles by targeting mRNAs for degradation or mediating translational repression.

We next used the polysome profiling assay to confirm that eIF4E2 participates in miRNA mediated translational repression.

Notably, let-7a overexpression reduced the total mRNA level of fLuc-LRE but not the control reporter RL, while downregulation of eIF4E2 did not affect the total mRNA level of either reporter (Fig. 4C), indicating that eIF4E2 mainly plays a role in the process of miRNA mediated translational repression but not the process of RNA decay.

In addition, it is subject to miRNA mediated translational repression [58].

These observations strongly suggest that miR-130a is acting through the A site of the FOG-2 3'UTR to mediate translational repression.

The G-quadruplex was modeled on one previously described to be present within the 5' UTR of NRAS RNA and shown to mediate translational repression [23].