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In first-generation FVIII products, the FVIII gene is expressed in Chinese hamster ovary cells and the cells are grown in culture media containing human albumin, bovine albumin and insulin to ensure production of sufficient amount of FVIII.
They grew well in media containing human growth factors or mouse growth factors, supporting the hypothesis that the cells contain only human DNA (Table 1).
We formulated a serum-free optimal media containing human serum albumin (HSA), L-ascorbic acid, and lipids (Table 1 and Figure S9) and found that this formula, used from day 2 4 to replace the FBS or human serum containing media produced 64.8±3.3% of hEB contracting by d15 of differentiation (Figure 1D).
The results confirm that various serum-free media lead to similar cell viability and recovery as compared to media containing human or calf serum.
Similarly, it was reported that 98%% of α-gal-positive MC38 colon carcinoma cells were killed by media containing human serum [ 16].
The results obtained in this single-center experiment confirm that the serum-free media used in the proficiency panel lead to background reactivity which was comparable to the background spot production induced by freezing media containing human AB serum, but also to other freezing preparations containing or lacking serum (FCS or CTL Cryomedium, respectively).
Similar(53)
After transferring the NPs into complete phenol red-free cell culture media containing 10% human serum (CPRFM), the spectra red-shifted 5 nm to a peak wavelength of 574 nm.
From the collected media containing secreted human adipocytes proteins 160 µg protein was separated by SDS-PAGE on pre-cast 4 12% polyacrylamide gradient gels (Invitrogene).
All values that were greater than the highest range within the standard curve were repeated after further dilutions with media containing 10% human AB serum.
Phosphoflow studies were run using aliquots of cryopreserved PBMC thawed and immediately diluted with RPMI complete media containing 5% human AB serum and 1% penicillin, streptomycin, and amphotericin (Omega Scientific, Tarzana, CA).
uNK CM or phenol red-free RPMI 1640 media containing recombinant human chemokine (C-C motif) ligand 2 (CCL2) (RnD; Cat #279-MC-010) was added to the bottom chambers.
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