Sentence examples for medium containing human from inspiring English sources

The human epithelial cells grown on the surface of the hydrogels detached within 10 min with preserving cell-to-cell connection by soaking in the medium containing human saliva at 5% (v/v).

The developed device consists of a microchamber made of polydimethylsiloxane produced by soft lithography and a perfusion system consisting of a reservoir, a dampener, a chamber, a shredder channel, and a peristaltic pump to constantly circulate cell culture medium containing human hepatocellular liver carcinoma cells.

After three washes, the amount of insolubilized MVs was measured by prothrombinase assay in standardized medium containing human coagulation factors (1.2 μM FII, 33.3 pM FVa, 11.2 pM FXa, 2.2 mM CaCl2).

N-hTERT keratinocytes were grown at 37°C under a 5% CO2 atmosphere in Epilife Medium containing human keratinocyte growth supplement HKGs (Cascade Biologics, Mansfield, UK).

We previously observed that the cytotoxic activity of the cyclin-dependent kinase inhibitor flavopiridol was substantially reduced in medium containing human serum vs. fetal bovine serum, with profound implications for effective clinical administration [23].

Cells were then stimulated in fresh FBS-free medium containing human recombinant IL-1β (10 ng/ml), TNF-α (10 ng/ml), IL-4 (10 ng/ml), IgE (1 or 10 µg/ml) or vehicle (medium alone) for time periods specific to experiments, as mentioned below.

DC/T cell cultures were carried out in complete medium: RPMI-1640 containing human AB+ serum (5%), L-glutamine (2 mM), and penicillin (100 U/ml)and streptomycin (100 µg/ml).

P. falciparum parasite strains 3D7, Dd2, and HB3 were routinely cultured in human O+ erythrocytes (NHS Blood and Transplant, Cambridge, UK) at 5% hematocrit in complete medium containing 10% human sera, under an atmosphere of 1% O2, 3% CO2, and 96% N2 (BOC, Guildford, UK).

TEFF (1 × 105) alone or in co-culture with different concentrations of TREG (i.e., 2.5 × 104, 5 × 104, 1 × 105) were then stimulated with CD3/CD28 beads (1 μl, Gibco) for 4 days in RPMI medium containing 10% human AB serum and 1% penicillin/streptomycin. CFSE dilution indicating cellular division of effector T cells was measured by flow cytometry (LSRFortessa, BD).

Cells were washed and cultured in RPMI 1640 medium containing 10% human AB serum (BioWest, Nuaille, France) and 10 U/ml interleukin-2 (IL-2, Roche Diagnostics, Mannheim, Germany).

In the presence of 40% human serum there was less than a 2-fold increase in the SPL7013 EC50 value compared to cultures infected in medium containing no human serum (Figure S2A).