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Cells incubated in serum-free media containing only mitomycin C migrated the least compared to 10% FBS (p = 0.032 10% FCS vs. Media + mitomycin C).
Media containing only xylitol was used in order to see if an adaptation was possible.
For f i, iWAT precursors were cultured in media containing only delipidated serum (grey bars), adipogenic cocktail (see the Methods section, white bars), or supplemented with EPA (turquoise bars) or ALA (purple bars).
For a d, iBAT precursors were cultured in media containing only delipidated serum (grey bars), or in the presence of insulin, T3 and ascorbic acid (ITA, white bars) or supplemented with eicosapentaenoic acid (EPA, tourquoise bars) or linolenic acid (ALA, purple bars).
Furthermore, cellobiose was assimilated faster than glucose when comparing media containing only glucose and media containing only cellobiose (Fig. 1a, e).
C. cellulovorans grew better in media containing cellulose and xylan than media containing only xylan or cellulose (Fig. 2).
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Chambers were placed in 12-well dishes and the top and bottom compartment were rehydreated with serum free and full serum media (DMEM containing only glutamine, sodium pyruvate, antibiotic/antimycotic and HEPES buffer) respectively for two hours at 37°C.
For all fluorescence measurements, we first remove the autofluorescence of the media and plasticware by subtracting the fluorescence of wells containing only media.
The following day NSCs were incubated in media containing DMSO only (1∶1000) or STS (50 nM) for 3 h at 37°C prior to processing.
As a vehicle control, Ba/F3 were resuspended in RPMI-complete media containing DMSO only.
When a diploid culture is plated on media containing cycloheximide, only cells that have lost the wild-type ACT2 allele can grow.
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