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The cell lines were grown in culture media containing heavy isotopes of lysine.
Bacteria were grown at 37°C in Luria Bertani (LB) medium containing ampicillin (100 µg/ml) and gradually adapted to grow in the presence of increasing concentrations of gold salts (from 0.1 mM to 10 mM), a common microbiological method for growing bacteria in media containing heavy metals and other toxic materials [40], [41].
In addition to the label-free identification of protein targets, we cultivated HepG2 cells in media containing heavy isotope-labeled arginine and lysine (ESI Fig. 7 †).
Proliferating myoblast (prolif) and differentiated myotube cells (diff) were cultured in media containing heavy arginine, and samples were collected over the course of 7 days to monitor new histone synthesis.
Two different populations of cells (tumor cells and normal cells) are cultured in vitro under similar conditions except that tumor cells are grown in media containing heavy isotope of an amino acid (e.g., C labelled arginine) and the normal cell line is grown in usual media.
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The consortia were able to grow on minimal media containing only heavy crude oil as carbon source.
Three different types of reported minimal salt media (M6, M7 and M8) were used to enrich bacterial consortia containing heavy crude oil as a carbon source.
EAFD is a hazardous waste containing heavy metals.
Fig. 4 Effect of metal ions on the growth of SXM in LB media containing different concentrations of heavy metal ions at 30 °C and 150 rpm.
Two populations of DG75 cells were grown in SILAC media containing either "light" or "heavy" (C6-labeled) arginine and lysine.
Cells were grown in 5 ml of synthetic complete media containing either 1 mM L-lysine (light) or 1 mM L-lysine [C6N2] (heavy) until stationary phase.
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