Sentence examples for media containing free from inspiring English sources

Following 24-h incubation, the old media containing free SLN and drug-loaded formulations were carefully aspirated and the cells were washed twice with PBS.

Furthermore, the occurrence of cavitation within the homogenizer and the production of free radicals during the nanodispersion preparation procedure along with the high surface area of the nanoparticles for exposure to this aqueous media containing free radicals are other possible causes of the degradation during sample preparation process and storage [2, 23].

The next day, old media was replaced with a fresh media containing free LPT and PLPT and incubated for 24 and 48 h, respectively.

Samples of culture media containing free or conjugated neurotrophic factors (final concentration 10 ng/mL, each factor) were kept at 37°C.

Culture media containing free [H]-leucine released upon intracellular degradation was collected, clarified by centrifugation at 500 g × 5 min and precipitated overnight at 4°C in a final concentration of 10% trichloroacetic acid.

Media containing preservative free antibodies directed against secreted proteins or the extracellular domain of membrane proteins is added to the cultures.

Similar results were obtain when exosomes were isolated from cytokine-treated MIN6B1 cells cultured in DMEM media containing exosome-free FCS (Additional file 6: Figure S5).

[1-14C]AA (NEN Life Science Products, Boston, MA; specific activity 53 mCi/mmol) was added to the cultures (1 µCi/ml per well; 1 well  = 2×106 cells) in serum-free culture media containing 0.5% fatty acid-free BSA.

Using a titre of 10/ml and an MOI of 10, MDA-231 cells were infected ~70-80 ~70-80uency in serum free and antibiotic free media containing polybrene (25 μg/mL).

Cells were then washed with HBSS and re-fed with serum free media or serum free media containing 15 nM dimethyl amiloride (Sigma).

The bottom wells were filled with serum-free media, serum-free media containing 10 ng ml−1 of G-CSF or serum-containing media (positive control).