Sentence examples for medium containing free from inspiring English sources

Next, the culture medium was removed and serum-free medium containing free Ats and Ats-loaded BSA NPs was added into the wells.

Moreover, as an extra control test, the encapsulated islets were cultured in a medium containing free PTX equivalent to the amounts in the co-encapsulated ones (400 µg/mL).

The drug loading and encapsulation efficiency was determined by separating the nanoparticles from the aqueous medium containing free lithium carbonate by centrifugation at 20,000 rpm at 4 °C for 60 min.

The culture medium was replaced with 200 mL of three groups of medium containing free GA (0, 0.125, 0.25, 0.5, 1, 2, and 4 μg/mL), GA-TiO2 nanocomposites, or GA-TiO2 nanocomposites with 180 s of UV irradiation, at 6 h of cell culture.

Aspirin encapsulation efficiency and loading capacity were studied by separating CS-NPs from the aqueous medium containing free drug by ultracentrifugation with 9,000 rpm at room temperature for 30 min.

The entrapment efficiency (EE) of the process was determined indirectly upon separation of the drug-loaded NPs (after dialysis) by ultracentrifugation at 25,000 rpm, 8°C for 30 minutes from the aqueous medium containing free DTX.

Next, culture medium was removed and re-added serum-free medium respectively containing free Dox and Dox-loaded NPs with different concentrations of Dox into cells for continuous incubation for 24 h.

Before treatment, cells were incubated for 24 h in serum free medium containing phenol-free DMEM supplemented with 1 mM sodium pyruvate, 4 mM L-glutamine, 1 100 nonessential amino acids, 1% insulin-transferrin-selenium-X supplement, 0.1% BSA, and antibiotics as described above.

Furthermore, the antioxidant properties of the isolate cultivated in medium containing and free of SeO32− ions were evaluated using DPPH scavenging and reducing power assays.

For all transgene experiments, cells were selected and grown in medium containing tetracycline-free serum.

H441 cells were maintained in F12/DMEM 50/50 medium containing tetracycline-free FBS (Clontech Laboratories), and transduced with retroviral supernatant in the presence of 4 μg/ml polybrene.