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Primer sets of S-P-MArch-0348-S-a-17 and S-D-Arch-0786-A-a-20 were used with the double-dye probe March-0515 for measuring the copy number of 16S rRNA from methanogenic archaea (Sawayama et al. 2004).
It is important to note that measuring the copy number of short nucleotide fragments could be, if necessary, performed by qRT-PCR rather than by more expensive sequencing.
We can then evaluate the lower limit of the target protein expression by measuring the copy number of the TEV plasmid.
It is important to note that measuring the copy number of short nucleotide fragments could be, if necessary, performed by qPCR rather than by more expensive NGS.
The advantage of gTOW is that we can indirectly estimate by how much a target protein can be overexpressed over the native level by simply measuring the copy number of the gTOW plasmid.
Viral stocks were obtained by CsCl gradient centrifugation; rAAV titer, determined by measuring the copy number of viral genomes in pooled, dialyzed gradient fractions, as described previously (16), was in the range of 1 × 10 to 1 × 10 genome copies/ml.
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In the next step, we measured the copy number of rDNA repeats in Sup mutants harboring pRRN5 plasmid.
The team then uses quantitative sandwich ELISAs that are calibrated to measure the copy number of proteins per cell.
To determine the persistence of the CART-HER2 cells, we used qPCR at serial time points to measure the copy numbers of CAR in the peripheral blood of mice in the experimental group.
In order to measure the copy number of rDNA repeat in Sup mutants, we performed pulsed field gel electrophoresis and subsequent Southern blotting using 5S rRNA gene as a probe (Fig. 3a).
Quantitative PCR was chosen as an independent method to measure the copy number of FCGR3B and was performed on 24 samples (Figure 3B).
Related(20)
measuring the transcript
measuring the material
measure the copy
measures the copy
measuring the season
measuring the temperature
measuring the center
measuring the inhibition
measuring the number
measuring the volume
measuring the efficiency
measuring the time
measuring the speed
measuring the space
measuring the phase
measuring the bounce
measuring the output
measuring the value
measuring the scope
measuring the beach
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