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Figure 1 Agreement and association between balance (balancepost-exsang). and CT performed in living animals (CT in vivo ) for measuring lung weight.

The aim of this study was to validate quantitative analysis of CT (against gravimetry) for measuring lung weight in pigs with or without pulmonary oedema.

Figure 2 Agreement and association between balance (balance post-exsang. ) and CT performed after exsanguination (CT post-exsang. ) for measuring lung weight.

The aim of this study was to evaluate the accuracy of quantitative analysis of thorax computed tomography (CT) for measuring lung weight in pigs with or without pulmonary oedema.

The tumor burdens were determined by measuring lung weight three weeks after the treatment.

This (final) lung weight was compared with the initial one, measured on pre-study CT taken at 0 cmH2ofof airway pressure (the degree of agreement between these two methods for measuring lung weight was assessed in five other piglets, as reported in Additional file 1).

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Moreover, quantitative analysis of thorax CT does not only measure lung weight but also characterises patients with acute respiratory failure [28], assesses distribution of ventilation [29], quantifies pulmonary inhomogeneities [30] and predicts response to therapy [31].

All mice were also given I-albumin intratracheally or intravenously to measure lung wet-to-dry weight ratio and lung epithelial and endothelial permeability as previously reported [ 5, 48].

HKII+/− mice also displayed a significant increase in LVED diameter (LVEDD; Fig 2F), and pulmonary oedema, as measured by lung weight over body weight 8 weeks after TAC, indicating the development of congestive heart failure (Fig 2G).

Lung CT images were manually outlined including only lung parenchyma and excluding bronchi and big intrapulmonary vessels and analysed with a dedicated software (Maluna 3.17, University Hospital of Göttingen, Germany) to measure lung gas content (i.e. functional residual capacity), lung weight and aeration distribution, as previously described [22].

To determine the effects of mechanical ventilation on changes of microvascular permeability and lung water in VILI, we measured lung EBD and the wet- to dry-weight ratio.

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