The presence of pulmonary edema was assessed by measuring lung water content 7 days after AMI: the lungs were dissected, weighed, dried at 60°C (24 hrs), and then re-weighed to calculate wet-to-dry ratio.
We did not evaluate the alteration of the alveolar capillary permeability from a more molecular point of view (such as expression of tight or adherent junction proteins), but only by measuring the lung edema following such alterations (wet-to-dry ratio and the CT scan analysis).
The gravimetric measure of lung edema poses considerable technical challenges, including evaporative loss and regional heterogenesity.
Thus, monitoring of EVLW might be a valid dynamic measure of lung edema at the bedside, supporting therapeutical decisions on patients with a compromised cardiopulmonary function.
WT-MSCs significantly reduced lung edema (c), as measured by wet-to-dry lung weight ratio (wet/dry) in the experimental groups in 24 h (ANOVA p < 0.05 [e]; *Dunnett's post hoc p < 0.05 vs. PBS. PBS n = 21; WT-MSCs n = 18; PTX3−/−-MSCs n = 10).
Therefore, we considered the "lethal ventilation" and lung edema as outcome measures.
In order to assess the effects of inhaled nitric oxide (iNO) in preventing early-onset lung edema from occurring after lung transplantation, we measured extravascular lung water (EVLW) in a group of lung transplant recipients who were at high risk for developing ischemia-reperfusion-induced lung injury.
To study the time course of lung edema formation we compared lung elastance measured by forced oscillations with invasive methods of lung edema detection (for example, wet dry weight ratio and histology).
The knowledge of reference values may be important to estimate, for example, lung edema and its distribution, which may be measured as excess tissue mass relative to normal subjects.
The knowledge of reference values may be important to estimate, for example, the quantity of lung edema and its distribution, which may be measured as excess tissue mass, relative to normal subjects.
