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Exact(6)
The optimum pH of the purified peroxidase was assayed by measuring its activity in standard buffer solutions of different pH values (pH 2.0 10.0) (Fluka).
The thermostability of xylanase was determined at 30 to 70°C by measuring its activity at a regular interval of 30 min up to 120 min.
The pH optima for xylanase activity was determined by measuring its activity in various buffers (0.05 M) such as sodium citrate (pH 3.0 to 6.0), sodium phosphate (pH 6.0 to 8.0) and glycine-NaOH (pH 8.0 to 10).
However, PP2A activity (measured indirectly as OA-inhibited phosphatase activity and directly by immunoprecipitating PP2A and measuring its activity) was markedly decreased in a dose dependent manner.
To test whether punicic acid could also affect neutrophil degranulation, human neutrophils were treated or not with punicic acid and MPO release was assessed by measuring its activity.
Importantly, pre-incubating complex I in high concentrations of metformin before measuring its activity in lower concentrations showed that metformin binding is reversible, and 24 h incubations followed by Blue native PAGE analyses revealed no loss of global structural integrity.
Similar(54)
As the fusion enzyme CR2-L-GDH involving both CR2 and GDH can be active towards both the substrate DKTP and the coupled co-substrate glucose, it would be necessary to evaluate the fusion enzyme by measuring its activities and kinetic parameters towards the two substrates, respectively.
Since the longest Rabex-5 construct is used as a reference for the mutants but has the weakest activity, it is important to measure its activity in an appropriate concentration range.
For example, it is not enough to state that you're going to add a "special buffer" to an enzyme and measure its activity.
In homogenates of NAWM from 10 subjects with progressive MS and 10 controls without central nervous system disease, we measured CK-B levels with an ELISA, and measured its activity with an enzymatic assay kit.
Although we analyzed MMP-12 protein expression and its localization, we did not measure its activity.
Related(20)
comparing its activity
measuring its production
assessing its activity
evaluating its activity
measure its activity
estimate its activity
measuring its draft
measuring its progress
measuring its exposure
measuring its success
measuring its relevance
measuring its pulse
measuring its performance
measuring its effect
measuring its climate
measuring its output
measuring its intensity
measuring its momentum
measuring its absorbance
measuring its range
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