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Since the longest Rabex-5 construct is used as a reference for the mutants but has the weakest activity, it is important to measure its activity in an appropriate concentration range.
For example, it is not enough to state that you're going to add a "special buffer" to an enzyme and measure its activity.
Although we analyzed MMP-12 protein expression and its localization, we did not measure its activity.
Extracted fractions were submitted for further radiochemical separation with the aim of purification of plutonium to measure its activity by alpha spectrometry.
Like haematological parameters, scintigraphic parameters use the capacity of the spleen to filter the blood of deviant cells and particles to measure its activity.
The major advantage that ISEs have over flame photometry is that ISEs do not measure the concentration of an ion; rather, they measure its activity.
Similar(52)
Isis has taken on board modern methods to measure its activities and to suggest areas for improvements.
The optimum pH of the purified peroxidase was assayed by measuring its activity in standard buffer solutions of different pH values (pH 2.0 10.0) (Fluka).
The thermostability of xylanase was determined at 30 to 70°C by measuring its activity at a regular interval of 30 min up to 120 min.
The pH optima for xylanase activity was determined by measuring its activity in various buffers (0.05 M) such as sodium citrate (pH 3.0 to 6.0), sodium phosphate (pH 6.0 to 8.0) and glycine-NaOH (pH 8.0 to 10).
However, PP2A activity (measured indirectly as OA-inhibited phosphatase activity and directly by immunoprecipitating PP2A and measuring its activity) was markedly decreased in a dose dependent manner.
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