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Exact(7)
None of the studied loci showed a correlation between the measured methylation levels and age of the DNA sample.
In phase I, we measured methylation levels at 1,505 CpG sites in treatment-naïve leukocyte DNA from 132 never-smoker PaC patients and 60 never-smoker healthy controls.
We fit a mixed-effects model to account for repeatedly measured methylation levels.
To study methylation changes on a genome-wide scale, we measured methylation levels along the chromosomes in the samples using a 1 Mb sliding window to smooth the distribution (Additional file 4).
Using this method, we measured methylation levels of 8 CpG units (13 CpGs), 14 CpG units (26 CpGs) and 10 CpG units (15 CpGs) in RiboPromoter, 18S and 28S target regions respectively.
It has been reported that some of the cell-free mitochondria present in plasma originate from platelets themselves [ 39], and so trace contamination with platelet-derived mitochondria and microparticles that contain mtDNA should not affect measured methylation levels, on account of the cells of origin being the same.
Similar(53)
Quantification of the measured methylation level was done after these known DNA standards and mixtures were processed in duplicate through all workflow steps starting from pre-amplification to sequencing.
The Methylation Ligation-dependent Macroarray (MLM), an array-based analysis, was designed in order to measure methylation levels of 58 genes previously described as putative biomarkers of cancer.
Both methods are highly sensitive and capable of measuring methylation levels.
We designed two independent primer panels to measure methylation levels at selected genomic regions.
In array-based methylation experiments, both Beta-value and M-value statistics are used as metrics to measure methylation levels.
Related(20)
estimated methylation levels
evaluated methylation levels
calculated methylation levels
demonstrated methylation levels
compared methylation levels
moderate methylation levels
assess methylation levels
measured expression levels
measured exposure levels
measured testosterone levels
measured mycotoxin levels
measured methylation differences
measured metal levels
measured methylation intensities
measured hemoglobin levels
measured glutamate levels
measured saliva levels
measured methylation patterns
measured illuminance levels
measured fluctuation levels
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