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Exact(6)
Firefly luciferase activity was measured and normalised to Renilla luciferase activity (Dual-Luciferase RePromegaAssay System; Promega).
After having measured and normalised the different sTRAIL variant concentrations, they were applied to pancreatic and control cancer cells.
For Figure 1 E-G, signal from individual ICM/Epiblast nuclei was measured and normalised to the intensity of DAPI signal.
Peak-to-peak amplitude of N9 (figure 3A, B) and amplitude of the first positivity of N20 (figure 3C, D) were measured and normalised to the prestimulus activity (calculated over a 100 ms period, excluding the stimulus artefact).
Transfected cells were steroid starved for 48 h and incubated with E2 (10 n) at indicated time points, and Luciferase activity was measured and normalised relative to renilla luciferase units using dual Luciferase reporter system as per the manufacturer's instruction.
For each reporter gene construct three (pC1L-series) or four (pC2L-series) bombardments were made, gene expression strength of both luciferases measured and normalised relative to the luciferase expression of p70Sruc (see below).
Similar(54)
The yield of DNA was measured using picogreen and normalised to 50 ng/μl before genotyping.
The cDNA levels of an internal control gene, the Actin gene, were measured and used to normalise the ATM cDNA levels.
The culture OD730nm was then measured and all samples were normalised to the same OD730nm and visualised.
Tissue TNF-α was measured by sandwich ELISA and normalised for total protein content.
The intensities of the bands were measured and MT-II mRNA expression was normalised with β-actin.
Related(20)
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