Sentence examples for calculated and normalised from inspiring English sources

Relative gene expression was calculated and normalised against internal controls (18S rRNA, ACTN4 and BAT1) [39], using the Pfaffl method [38].

Test/reference ratios were then calculated and normalised to the median ratio of the autosomal chromosome clones.

For each GC/FID spectrum, the areas of the assigned peaks were calculated and normalised to their total area.

The number of synonymous and non-synonymous SNPs either side of this location was calculated and normalised to the domain size.

After 24 h, the mean density of cells in the scratched area was calculated and normalised against the migration induced by growth factor alone.

The percentage of methylated reference (PMR) was calculated and normalised against β-actin to account for variability in the amount of input bisulfite-treated DNA.

From the load and displacement range, the stiffness was calculated and in situ normalised to its value after 1000 cycles.

The mean signal intensity for each treatment dose was calculated and these values were normalised to the mean signal intensity for the element in the intra-animal control sample.

The relative expression of the target gene was calculated as arbitrary units and normalised against the expression level of spiny dogfish β-actin, a housekeeping gene.

For the unique genes obtained, additivity; α = (wild-domesticated)/2 and dominance parameters; δ = (wild + domesticated)/2-hybrid were calculated from normalised intensity values and α and δ/α were plotted using the ggplot2 package [ 57].

The relative quantification was calculated using the ΔΔCt method and normalised to the control group.