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As an alternate approach to measure cell death, the experiment was repeated in 6 well plates and cells were harvested 48 hours after plasmids transfection.
To measure cell death rates, cells were plated in 96 well plates at 1×104/well, and then treated with fatty acids or FASN inhibitors for 24 hrs, followed by adriamycin for different periods of time.
Plaque reduction assays also measure cell death.
It is used to detect and measure cell death.
Our results indicate FSAP activation to be a useful tool to measure cell death in circulation.
Cells were washed with PBS and analyzed by flow cytometry to measure cell death.
Similar(38)
Furthermore, the OD measurements may not have accurately measured cell death.
Currently, models using tissue culture methods for measuring cell death in clonal cell lines, and in skin-equivalent models, have been accepted by regulators.
We measured cell death by quantifying extracellular LDH activity in cells lines expressing WT and G93A SOD1, treated with MG132, L-NAME or both (Fig. 7H).
As a complementary method to assess biocompatibility we used the AK assay that measures cell death through the release of adenylate kinase via a luminescence read-out.
The M65® ELISA assay, which detects all forms of CK18, measures cell death due to both apoptosis and necrosis (CK18 intact and fragment), while the M30 Apoptosense® ELISA assay specifically measures apoptosis (the caspases-generated CK18 fragment, CK18-Asp396).
Related(20)
measure cell mortality
measuring cell death
measure cell size
measure cell viability
measure cell toxicity
measure cell membrane
measure cell traction
measure cell survival
measure cell contraction
measure cell adhesion
measure cell migration
measure cell invasion
measure cell apoptosis
measure cell cycle
measure cell motility
measure cell concentration
measure cell proliferation
measure cell fusion
measure cell envelope
measure cell density
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