Then, the medium was removed, PBS was added, ASMCs were detached from the plate, the cell's area was measured, cell contraction was provoked by the addition of ACh (10-3 M) or CCh (10-5 M) for 5 min and the cell's area was measured again.
Collagen gel deformation was measured with a cell contraction assay (Cell Biolabs, BioCat GmbH, Heidelberg, Germany). 1 × 10 cells in 650 μl collagen gel lattice were cultured for 2 days.
In the study of cell contraction, H9c2 cells were measured by the change in the planar surface area, as described previously [ 31].
However, aberrant cell contraction can result in pathological contracture.
Cell contraction and rupture of cell-cell contacts results in gap formation and leak [7], [12] [14].
Interfering with actomyosin contraction demonstrated that interfering with cell contraction can also diminish cell cohesion.
The effects of disrupting actin, microtubules or intermediate filaments on cell-cell interactions were assessed by measuring cell sheet cohesion and contraction.
Single-cell contraction was measured by video edge detection, and recordings were made under basal conditions and after administration of 100 µM ouabain [27].
Cell-cell contraction and rupture of cell-cell contacts result in capillary leak.
First, in order to directly measure putative contraction of the neuronal actomyosin cortex, we measured the cell cortex rigidity of isolated CB1R-expressing Neuro2A cells before and after cannabinoid treatment, by using atomic force microscopy (AFM).
