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Currently, models using tissue culture methods for measuring cell death in clonal cell lines, and in skin-equivalent models, have been accepted by regulators.
A novel dye-based method for measuring cell death in image-based screens is presented.
Thus, instead of measuring cell death to assess modulation of rituximab activity by RF, we measured deposition of C3b on the B cell surface.
When measuring cell death, p53wt→ p53−/− HCT116 responded to 5-FU similar to their p53wt/wt Hcounterparterpand, and correspondingly, PHD1 silencing resulted in an increase in parp cleavage upon 5-FU treatment compared to control cells (Fig EV1D).
We then looked at the toxicity of different HDACi in U937-B8 cells 1 week after removal of vorinostat from the culture media, by measuring cell death by PI staining.
We tested whether caspase cleavage of GRASP65 is required for apoptosis by measuring cell death (cleaved PARP) in HeLa cell lines stably expressing wild-type or caspase-resistant GRASP65 GFP.
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Furthermore, the OD measurements may not have accurately measured cell death.
At the end of the assay 7AAD (which binds to DNA and can only enter membrane-compromised cells) is added to measure cell death.
We measured cell death by quantifying extracellular LDH activity in cells lines expressing WT and G93A SOD1, treated with MG132, L-NAME or both (Fig. 7H).
As an alternate approach to measure cell death, the experiment was repeated in 6 well plates and cells were harvested 48 hours after plasmids transfection.
As a complementary method to assess biocompatibility we used the AK assay that measures cell death through the release of adenylate kinase via a luminescence read-out.
More suggestions(15)
measuring cell density
measuring cell apoptosis
measuring cell proliferation
measuring cell voltage
measuring percent death
measuring cell uptake
measuring cell response
measuring cell viability
measuring cell surface
measuring cell type
measuring cell adhesion
measuring cell cycle
measuring cell size
measuring cell b
measuring cell number
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