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The japonica varieties had a mean response index of 2.18 cm and ranged from 0.64 to 3.62 cm, while the indica varieties had a mean response index of 1.66 cm and ranged from 0.55 to 2.54 cm.
These accessions had a mean response index of 2.09 cm and ranged from 0.8 to 3.04 cm (Additional file 3: Figure S1a).
Second, the mean response index of Or42a − larvae to ethyl butyrate was elevated compared to wild type at 10−2 (ANOVA, Tukey Kramer, p = 0.0007) (Fig. 1B).
The behavioral response threshold is defined in this study as the lowest concentration of odor where the mean response index of subject larvae is statistically different from the mean response index of negative control larvae, Orco −; all statistical comparisons reported in this section are relative to Orco − larvae.
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(G ) Mean response indices ± SEM of FS neurons were also significantly different (Wilcoxon signed rank test) between facial touch and off-response windows.
(D ) Mean response indices ± SEM of RS neurons during facial touch and off-response were significantly different (Wilcoxon signed rank test).
Mean response indices of FS neurons, on the other hand, showed a robust positive modulation to all calls and to individual call categories.
Mean response indices for Au1 RS neurons showed that the overall inhibition due to touch was reversed after the touch episode (p = 0.0329).
The mean response indices ± SEM to either all calls or the major call categories also reveal little or no overall modulation (right).
To rule out this potential confound, we applied bootstrapping analysis (see 'Materials and methods') to compute the mean response indices for our data set and plotted the frequency distribution histograms as before.
(D ) Almost all FS neurons were also significantly upregulated by USVs (left, Wilcoxon signed rank test) and this was evident in their mean response indices ± SEM to all calls and to the various call categories (right).
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