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Treatment with KOS GSE also alleviated the reduction in mean dendrite length significantly (Figure 2C).

Finally, the mean dendrite length was calculated by dividing the total dendrite length by the total cell number.

The mean dendrite length was quantified using ImageJ software as follows, in which an image analysis method for tumor vessel architecture [32] was applied with modifications.

Morphometric analysis also demonstrated that the mean dendrite length in neurons treated with KOS GSE was longer than those treated with glutamate alone (Figure 3B).

A morphometric analysis indicated that the mean dendrite length of stressed neurons was reduced by 50% as compared to the untreated control (Figure 2C).

However, as the cell density affects the dendrite length significantly, and the high cell density images tend to underestimate the mean dendrite length due to overlap, images with a cell density of ∼50±15 cells/mm2 were used for the following morphometric analysis.

Similar(54)

Some of the stem dendrites could be followed over wide distances with a mean dendritic length of 557.1 ± 275.0 μm (minimum 159.7 μm, maximum 941.7 μm; see also Table  1).

The dendrite length was expressed as the number of dendritic trees that exceeded the limit of the ROI (number of dendrites > 50 μm).

In addition to the shortened primary dendrite length, however, the KI mice also showed a decrease in spine number.

Cells were fixed and immunostained for MAP2 (Figure 3A) to identify surviving neurons and analyze dendrite length.

Percentages of detached dendrites were calculated by the ratios of detached vs total dendrite length.

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