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The mean locus length was 508 bp.
Loci ranged in length from 424 2156 bp, with a mean locus length of 1374 bp.
The mean locus length was 1362 bp (range 555 3672 bp), for a total targeted length of 378,553 bp.
After applying coverage criteria, trimming all loci to the length of the second shortest sequence and removing all internal gapped characters, the mean locus length was 534 bp with a minimum and maximum lengths of 250 bp and 1458 bp, respectively (Note that loci with trimmed alignments shorter than 250 bp were removed).
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The mean locus (unigene) length and N50 value were calculated using a transcript length list.
In the 'Sachinoka' map, 34 LGs were developed, totalling 2166.6 cM in length, with a mean locus density of 3.90 cM locus−1.
In the 'Akihime' map, 33 LGs were developed, with a total length of 951.4 cM and a mean locus density of 2.99 cM locus−1.
The minimum predicted locus length was 30, while the maximum predicted locus length was 550.
We refer to this correlation as the locus length bias.
Mean length: Mean length of assembled sequences.
We calculated phylogenetic informativeness (PI) [ 40] per nucleotide site for the three UCE and the 10-gene data sets with the software package TAPIR [ 41] (http://faircloth-lab.github.com/tapir/), a parallelized version of PhyDesign [ 42].> -wrap-foot> Summary of number of loci, length of matrix, mean PI, number of data partitions and type of analyses for the four data sets used in this study.
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