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As such, eBURST and a maximum parsimony minimum spanning network analyses were carried out.

Based on the alignment we created multiple phylogenomic trees (maximum parsimony, minimum evolution, neighbor joining) including 100 bootstrap replicates.

The evolutionary history was inferred using the maximum parsimony, minimum evolution (ME), neighbor-joining (NJ) and unweighted pair-group method with arithmetic mean methods.

The several chloroplast GPx proteins clustered with different proteins in maximum parsimony, minimum evolution and neighborhood joining trees (Fig. 14B; Additional files 9 and 10).

Phylogenetic analyses were conducted with MEGA version 3.1 software [ 86] using maximum parsimony, minimum evolution, and neighbor-joining methods with the Poisson-correction for multiple amino acid substitutions.

Neighbor-joining, maximum parsimony, minimum evolution and UPMGA methods were used to reconstruct phylogenies, with MEGA4 [ 30], and the following parameters: pairwise deletion, Poisson correction and uniform rates among sites.

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Similar(52)

Four methods were employed, Neighbor-joining (NJ), UPGMA, Maximum parsimony, and Minimum evolution.

Instead, the strains branched deeply within the Proteobacteria as shown in a phylogenetic tree created by maximum likelihood [19], Figure 3. Very similar tree topologies were found by neighbor-joining, maximum parsimony or minimum evolution methods using MEGA3 [20](data not shown).

Whatever the method used (maximum likelihood, maximum parsimony or minimum evolution), no clear evidence of phylogenetic structure was observed, as illustrated with the maximum likelihood analysis.

SAG: Culture Collection of Algae at Göttingen University, Germany (Sammlung von Algenkulturen der Universität Göttingen, Göttingen, Germany); ML: Maximum likelihood; MP: Maximum parsimony; ME: Minimum evolution; NJ: Neighbor joining.

Four methods were used to infer phylogenetic relationships: maximum likelihood (ML), maximum parsimony (MP), minimum evolution (ME), and neighbor joining (NJ).

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