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Factors related to increased age at first calving were increased difficulty of delivery, antibiotic treatment of sick calves, increased amount of milk or milk replacer fed before weaning, reduced quality of forage fed to weaned calves, maximum humidity, mean daily temperature, and maximum ammonia levels in calf housing areas.

These two mutant strains, along with mutant strains B and C, produced higher maximum ammonia levels compared to wild type.

Strains were identified among these mutants that had a faster doubling time, produced higher maximum ammonia levels (enzyme assay) and more oil (Sudan Black assay), and had higher maximum soluble protein levels (Bradford assay) than wild type.

The maximum ammonia levels for four of the mutant strains, B (1.04 g/L), C (1.60 g/L), E (1.31 g/L), and F (1.01 g/L) were higher than for the wild-type strain (0.96 g/L).

The maximum ammonia levels reached for wild-type strain and mutant strains B2, A, B, and E were 0.96, 0.91, 0.96, 1.04, and 1.31 g/L, respectively, at 120 h, and for mutant strains C, D, and F were 1.60, 0.98, and 1.01 g/L, respectively, at 144 h.

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On K. marxianus-treated CWM, the maximum ammonia level of mutant strain F was 0.12 g/L at 7 days (Fig.  6).

For 100 mL synthetic ammonia solution, the optimum pH and MW energy output level for ammonia removal were 11 and 78 KJ and the maximum ammonia removal efficiency predicted for the synthetic solution is 76.3%.

Ammonia levels were similar in both reactors.

Ammonia levels in water entering most wetlands were considerably higher than ambient (i.e., riverine) levels; concentrations were reduced by as much as 92% in the wetlands and retained at a maximum rate of 166 g N m−2 year−1.

Furthermore, carbon dioxide and hence pH influence unionised ammonia levels.

Fish exposed to toxic levels of ammonia cannot excrete ammonia efficiently; as a result, ammonia levels in blood and tissues increase along with pH levels, therefore affecting enzyme activity (Lawson 1995).

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