Sentence examples for maximal transfection efficiency from inspiring English sources

These constructs were transfected into HEK293 cells to ensure maximal transfection efficiency and protein abundance and each resulted in the expression of proteins of the expected sizes when probed with antibodies to detect the triple-FLAG tag at their N-termini (Fig.  1a).

Upon an optimal balance between membrane activity and cytotoxicity, maximal transfection efficiency was achieved which outperformed commercial reagent Lipofectamine™ 2000 (LPF2000) by 3 6 folds.

Among all CR tested, maximal transfection efficiency and reasonable cytotoxicity for the aims of the present work were obtained with the minimal dose of liposomes corresponding to lipoplexes at CR5 (Fig. 1B).

Schizonts for barcode counting experiments were produced in female Wistar rats (200 250 g) to achieve maximal transfection efficiency.

Similarly, out of the four, the Lipofectamine 2000 transfection reagent promoted maximal transfection efficiency in HeLa Cells (43.7 ± 2.7%) compared to the other reagents (Fig. 5); X-tremeGENE HP resulted in the second highest CFP expression (33.9 ± 1.5%).

So as to get the best curative effect based on UTMD, future studies should go on to explore the UTMD technique to both obtain maximal transfection efficiency and minimal side effect.

As represented in the top of Fig. 4, the Lipofectamine 2000 transfection reagent promoted maximal gene transfection efficiency (F/T ratio) in HeLa cells (42.5 ± 5.9%) compared to the other three reagents.

Transfection efficiency was maximal after 108h, as determined by semi-quantitative RT-PCR.

To achieve the maximal therapeutic effects, nucleofection reagents were modified to improve gene transfection efficiency in primary cell cultures of fibroblasts (details described in Materials and Methods).

Maximal expression was achieved at 48 h post transfection and the transfection efficiency was approximately 40% as detected by green fluorescent protein (GFP) under microscopic observations.

Maximal expression was achieved 24 to 48 h post transfection with a transfection efficiency of approximately 70% at 48 h, as monitored by staining for myc expression.