Exact(38)
HEK293 cells, which exhibited the highest transfection efficiency, were transfected with pcDNA/WTH3 or pcDNA3.1 to perform flow cytometry assays.
It is noteworthy to say among three mentioned constructs 2RMPG-2H1 showed the highest transfection efficiency.
The highest transfection efficiency was obtained using TkBPVTH plasmids (5 μg).
Arg10-PEG-BDB showed the highest transfection efficiency, without severe toxicity to cells.
The (AB3 3 architecture micelle copolymer exhibited the highest transfection efficiency, about 2.5 times higher than PEI.
Especially, the corresponding LPCM displayed the highest transfection efficiency in HeLa cells (P < 0.05) when DOPE grafting ratio reached up to 30%.
Similar(22)
To achieve higher transfection efficiency, cells were transfected twice and screened with G418 (Gibco).
Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency.
In both HEK293 and COS7 cells, these polyrotaxanes show low cytotoxicity and high transfection efficiency.
In vitro experiments show that these polymers have lower cytotoxicity and higher transfection efficiency than 25 KDa PEI.
Results show that FuGENE 6 Transfection Reagent exhibits very low cytotoxicity, delivers high transfection efficiency and generates few nonspecific side effects with minimal alteration in gene expression.
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