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To maximize transfection efficiency during the 4 h incubation, the serum complement was reduced to 0.5%.
DNA amounts, transfection reagent amounts, and transfection duration were optimized to minimize toxicity and maximize transfection efficiency.
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To maximize transfection efficiencies, A549 cells were transfected using the Amaxa Nucleofector II system (Lonza, Germany) as described by their cell type-specific protocols.
To maximize the transfection efficiency, 3T3-L1 preadipocytes were transfected using an OneDrop Microporator MP-100 (Digital Bio, Seoul, South Korea).
This is a crucial aspect to maximize the transfection efficiency of catanionic vesicles.
At 24 h after transfection, cells underwent RT-qPCR and Western blot to verify transfection efficiency.
The transfection efficiency was confirmed by Western blot (Fig. S2).
The transfection efficiency was investigated by flow cytometry.
Transfection efficiency was normalized to β-Gal activity.
Results showed that it was proportional to cell transfection efficiency.
Figure 4 In vitro transfection efficiency of EGFP-loaded NPs.
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